New Comfort Zone in a spray form makes it great for use on the go (a limitation of the plug-in version, sold separately). The concentrated, veterinarian-approved Comfort Zone disperses dog-appeasing pheromone, which mimics a natural comforting pheromone produced by dogs. It helps to calm dogs or puppies in stressful situations and reduces stress or boredom behaviors including barking, whimpering, whining, and chewing. Calms dogs during thunderstorms and fireworks or when left home alone. Also reduces or eliminates separation anxiety. D.A.P. mimics a natural reassuring pheromone produced by female dogs when nursing. Product is odorless and non-toxic, does not affect humans. Not a drug or tranquilizer. Ingredients: Canine "appeasing" pheromone 2%, isopropanol filled to 60 ml. To use: spray on bed, bandana, in car or crate 15 minutes before exposing dog to treated area. Use 8-10 sprays every 2 hours. DO NOT SPRAY DIRECTLY ON DOG. One 60 ml spray bottle contains approximately 50 applications. D.A.P. is a registered trademark of Ceva Santé Animale